Functional Assays

The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features: • Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. • Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time • High-throughput: simple homog

The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Uric Acid Assay Kit is a quantitative colorimetric uric acid determination at 590 nm.

Iron is the most abundant transition metal element within organisms, and it participates in various physiological activities. Recently, free iron in living cells has attracted attention because its high reactivity may be related to cellular damage or death. Free iron exists in its stable redox states, namely ferrous ion (Fe2+) and ferric ion (Fe3+)). In living cells, understanding the behavior of Fe2+) is considered more important than understanding that of Fe3+) because of the intracellular reductive environment, metal transporters, and the water solubility of Fe2+). FerroOrange has no no chelating ability. FerroOrange and Fe2+ react irreversibly, which is different from the detection principle of calcium-iron probes such as Fluo-3.

- Selective measurement of Lipid Peroxide - Less cellular photo-damage - Applicable for microscopy and FCM analysis Liperfluo, a perylene derivative containing oligooxyethylene, is designed and exclusively developed by Dojindo for the detection of lipid peroxides. Liperfluo emits intense fluorescence by lipid peroxide specific oxidation in organic solvents such as ethanol. Among fluorescent probes that detect Reactive Oxygen Species(ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo are 524 nm and 535 nm, respectively, photo-damage and auto-fluorescence from the sample can be minimized. The tetraethyleneglycol group linked to one end of diisoquinoline ring helps its solubility and dispersibility to aqueous buffer. Liperfluo’s oxidized form is nearly nonfluorescent in an aqueous media and emits a strong fluorescence in lipophilic sites such as in cell membranes.

-High sensitivity for Fe2+ detection in mitochondria -Suitable for live cell imaging. It is reported that iron is the most abundant transition metal element within an organism and shows various physiological activities. Recently, free iron in living cells is getting attention because its high reactivity is suggested to be related to cellular damage or death. Free iron exists in its stable redox states, ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, it is considered that understanding the behavior of Fe2+ is more important than that of Fe3+ because of the intracellular reductive environment, metal transporters and water solubility of Fe2+. Mito-FerroGreen is a novel fluorescent probe for the detection of ferrous ion (Fe2+) in mitochondria where Fe-S clusters and heme proteins are synthesized, and enables live cell fluorescent imaging of intracellular Fe2+. Mito-FerroGreen has no no chelating ability.