Cell-based Assays

Find our optimized NADPH regeneration system and catabolism buffer to pair with your in vitro assays using recombinant enzymes and subcellular fractions. RapidStart™ is a versatile system that supports extended metabolism of test articles by NADPH-dependent drug-metabolizing enzymes in subcellular fractions for in vitro drug metabolism assays. The system is based on Glucose-6-Phosphate DeHydrogenase (G6PDH) and Glucose-6-Phosphate (G6P), with reduction of NADP+ to NADPH. NADPH is a cofactor required to support reactions by drug-metabolizing enzymes such as cytochrome P450 (CYP) and flavin-containing monooxygenases (FMO). Assays using microsomes, S9 and recombinant CYPs (Bactosomes) can be supported with RapidStart™ to support the long-term metabolism of test articles by these enzymes by ensuring that NADPH is not the limiting reagent.

Kit Contents: OrisTM-compatible 96-well plates, black with clear bottom (4) OrisTM Cell Seeding Stoppers, 4 packs of 24 (sufficient for 96 tests) OrisTM Detection Mask (1) OrisTM Stopper Tool (1)

Quantification of Reduced Glutamine - Easy extraction of cell Lysate - Used to determine intracellular levels of Glutamine Glutamine, a source of α-ketoglutarate (one of the tricarboxylic acid (TCA) cycle intermediates), is an important substance used for energy production and the syntheses of nucleic acids and other amino acids. Glutaminolysis, the process by which glutamine as a substrate is converted to α-ketoglutarate, is upregulated in cancer cells. A study reports that glutaminolysis has largely contributed to scavenging of reactive oxygen species and reduction of oxidized glutathione. The Glutamine Assay Kit-WST can quantify glutamine, a substrate of energy metabolism. Our Glutamine Assay Kit-WST is designed to quantify glutamine as a metabolite. This kit allows you to quantify glutamine present in culture medium or intracellular glutamine by WST reduction reaction. The lowest concentration of glutamine which can be quantified is 5 μmol/l. This kit can be used with 96-well micro

Quantification of Reduced Glutamate ~Features~ - Easy extraction of cell Lysate - Used to determine intracellular levels of Glutamate

Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. The different actin isoforms can show distinct cellular and sub-cellular expression and localization. It has been demonstrated that different isoforms have subtly different biochemical properties in vitro which supports functional diversity within isotypes in vivo. Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. BK001, the actin binding protein spin-down assay kit provides G- or F-actin (rabbit skeletal muscle origin) plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. Actin binding occurs when there is an affinity for any site of actin. F-actin binding can

Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. The different actin isoforms can show distinct cellular and sub-cellular expression and localization. It has been demonstrated that different isoforms have subtly different biochemical properties in vitro which supports functional diversity within isotypes in vivo. Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. BK003, The Actin Polymerization Biochem Kit is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be used to follow polymerization over time. Also, by us

BK004P, The tubulin polymerization assay is based on an adaptation of the original method of Shelanski et al. and Lee et al. which demonstrated that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The resulting polymerization curve is representative of the three phases of microtubule polymerization, namely nucleation, growth, and steady state equilibrium. The tubulin used in this assay (Cat. # HTS03) has been purified from porcine brain and consists of approximately 97% tubulin and 2% microtubule associated proteins (MAPs). This assay has been designed to give a standard polymerization yielding approximately 40-45% polymer mass. This allows the polymerization reaction to be highly sensitive to both polymerization enhancers (e.g. paclitaxel, MAPs) and polymerization inhibitors (e.g. nocodazole). The BK004 polymerization assay is suitable for screening large numbers of tubulin ligands and primary libraries. At 5 μM paclita

Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. The different actin isoforms can show distinct cellular and sub-cellular expression and localization. It has been demonstrated that different isoforms have subtly different biochemical properties in vitro which supports functional diversity within isotypes in vivo. Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. BK005 is a fluorescence format F-actin Visualization Biochem Kit. The kit utilizes fluorescently labeled phalloidin to selectively stain filamentous actin at nanomolar concentrations. They are the reagent of choice for F-actin staining of fixed cells for several reasons: 1. Bind in a stoic